Screening of reference genes in Siniperca chuatsi for qRT-PCR analysis. Reference genes for quantitative analysis on Clonorchis sinensis gene expression by real-time PCR.
Comparative Biochemistry and Physiology Part B, Biochemistry & Molecular Biology, 2001, 130(3):281-289 Control genes in quantitative molecular biological techniques:The variability of invariance. Ammonia pollution characteristics of centralized drinking water sources in China. Water quality assessment by pollution-index method in the coastal waters of Hebei Province in western Bohai Sea, China. Nitrogen stable isotope ratio in the Manila clam, Ruditapes philippinarum, reflects eutrophication levels in tidal flats. Beijing:Ministry of Ecology and Environment of the People's Republic of China, 2019(in Chinese) 北京:中华人民共和国生态环境部, 2019 Ministry of Ecology and Environment of the People's Republic of China. Sustainable aquaculture:Developing the promise of aquaculture. Journal of Fishery Sciences of China, 2016, 23(4):729-758(in Chinese)įrankic A, Hershner C. Species composition, non-fed rate and trophic level of Chinese aquaculture. philippinarum under ammonia nitrogen stress. Final results indicated that the expression stability order of the six candidate reference genes in the hepatopancreas were Actin>18S>CyPA>Ubi>Tubu>EF-1α, which means Actin is the most stable reference gene and provides a reliable basis for further study on expression levels of other genes in the hepatopancreas of R. GeNorm, NormFinder, BestKeeper and RefFinder statistical softwares were used to evaluate the expression stabilities of six candidate reference genes, namely beta-Actin ( Actin), elongation factor 1-alpha ( EF-1α), beta-tubulin ( Tubu), 18S rRNA ( 18S), ubiquitin ( Ubi) and cyclophilin A ( CyPA). In this study, clams was exposed to two different concentrations of ammonia nitrogen for 14 d, and the cDNAs of hepatopancreas at different time points were used as qRT-PCR templates. However, there's no related report about the expression stability of reference genes in hepatopancreas of R. With the further toxic study of ammonia nitrogen on clam Ruditapes philippinarum, it is necessary to accurately evaluate the expression profiles of related genes in clam hepatopancreas. **Sea water with Bull kelp can be substituted with regular water mixed with a pinch of sea salt and 1 sheet of nori.It is necessary to screen a suitable reference gene to quantify the expression levels of targeted genes by fluorescence quantitative PCR technology. Surround with deep fried clam strips and garnish with scallions. Drain on paper towel lined plate, then salt immediately with coarse salt. Drop into wok and deep fry 2 to 3 minutes until crisp and brown.
![ming the clam ming the clam](https://www.oldest.org/wp-content/uploads/2021/10/quahog-clam.png)
Toss the cabbage in this mixture.įor the Fried Clam Strips: Heat the oil in a wok or other deep frying vessel to about 350 degrees F.ĭip the clam strips, first in flour, then egg, then panko, to coat. Cover the steamer and cook until heated through, about 10 minutes.įor the Cabbage Slaw: Whisk together the olive oil, lemon juice, lime juice, salt and pepper. Bring the water to a boil and place the stuffed clams in the steamer. Pour the sea water mixed with the kelp into a wok fitted with a steamer.
![ming the clam ming the clam](https://pics.me.me/meet-ming-the-507-year-old-clam-the-oldest-recorded-animal-in-7109108.png)
Fold the mixture into the rice with the chopped crabmeat.ĭivide the filling among 6 razor clam shells. Season with salt and pepper, to taste, and mix lightly. Add the chopped razor clam meat, scallions, and cilantro. When the oil shimmers, add the garlic and ginger, and stir-fry about 2 minutes until soft. Add 2 tablespoons of oil and swirl to coat the pan. For the stuffed clams: Heat a wok over high heat.